In case of doubt, gonads were considered as undifferentiated and sex was not assigned. Detection of regulatory variation in mouse genes. Leeb M, Wutz A. Journal of genomics. Based on the AaegL4 assembly, the genomic region associated with a significant LOD score ranged from
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. See text for further details and references. Only female mosquitoes were genotyped in this cross because it was originally designed to map quantitative trait loci QTL underlying dengue vector competence, a female-specific trait.
All metamorphs and froglets were dissected under a binocular microscope to identify phenotypic sex based on gonad morphology. Regardless of the differences in experimental protocols pooled WGS vs. A potentially useful avenue of research into evolution of dosage regulation would be to compare X- or Z-linked ohnologs to their autosomal copy.
Allele-specific expression Some genes are normally expressed from a single allele.
The same markers showed high male—female genetic differentiation. In this case, the region of high LD that we detected around the sex-determining locus may simply be the consequence of its location in the centromeric region. Analyses of genome-wide variation in the unrelated laboratory strain Liverpool used to generate the current reference genome sequence, as well as in wild Ae.
Towards mosquito sterile insect technique programmes: exploring genetic, molecular, mechanical and behavioural methods of sex separation in mosquitoes. In sticklebacks recently evolved sex chromosomes allowed the definition of two main strata along the X, with the older strata showing evidence of upregulation in males and overexpression in females at least for a subset of genes [ 98 ].
In contrast to mammals, birds have extensive sex-bias in gene expression due to incomplete dosage compensation [ 10 ]. Long-read sequencing technology was recently used to improve the assembly of repeat-rich Y chromosome sequences in Anopheles mosquitoes Hall etal.
The position of sex-determining genes in the chromosomes of Rana nigromaculata and Rana brevipoda. Translating dosage compensation to trisomy Dosage compensation in Drosophila. Perrin N. Proc R Soc B :