Of interest. We have included insets in the SMCGFP panels of Figure 4G to magnify specific chromosomal regions from diplotene nuclei of WT and wapl-1 mutants, facilitating the comparison of axial element organization in the two genotypes.
Using the C. Condensin II complex.
Promotes mitotic proliferation in the distal germ line. Release of a subset of cohesin complexes at anaphase I then allows separation of homologs, thereby accomplishing the reduction in ploidy necessary for sexual reproduction. In each of the two daughter cells the chromosomes condense again into visible X-shaped structures that can be easily seen under a microscope.
Zinc-finger protein. Wapl induces cohesin dissociation from DNA throughout the mitotic cell cycle, modulating sister chromatid cohesion and higher-order chromatin structure. Mutations result in high levels of resected ssDNA and meiosis production of sex cells in Crawley germline apoptosis.
Unlike in normal cell division mitosisthe genetic material of the original parent cell is divided up twice. Associates with short arm of metaphase I bivalents and between sister chromatids at metaphase II Heterozygous null mutants show altered distribution of DSBs and COs, increased CO frequencies, and increased pachytene axis length Section 5.
Furthermore, the formation of this structure is specific to the cells in which they reside spermatocytesrather than a feature of either the X or the Y chromosome Handel
A two-part list of links to download the article, or parts of the article, in various formats. In contrast, late pachytene nuclei of wapl-1 syp-1 double mutants contained fewer HTP-3 tracks, which also appeared bulkier Figure 4C. Summary C.